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Evaluation of Genetic Diversity in Sisymbrium irio Germplasm Using ISSR Marker | ||
| فناوری زیستی در کشاورزی | ||
| Article 9, Volume 7, Issue 1, June 2016, Pages 71-78 PDF (554.2 K) | ||
| Document Type: research | ||
| DOI: 10.22084/ab.2016.1791 | ||
| Authors | ||
| Hedayat Bagheri* 1; Adel Jafari2 | ||
| 1Assistant Professor, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan | ||
| 2M.Sc. Student, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan | ||
| Abstract | ||
| Sisymbrium irio or bitter flixweed is important among the medicinal plants. The human population overgrowth and absence of a comprehensive management of natural resources caused a fast decrease in most valuable indigenous germplasm. Management of genetic diversity of native plants is important in conservation programs. Using molecular techniques, especially ISSR, one can study genetic diversity and analyze the genetic variation. In this study the genetic diversity of 33 populations of S. irio has been investigated by Inter Simple Sequence Repeat (ISSR) marker. In total, 183 sites were investigated. 11 ISSR primers in this study showed 100% polymorphism. The average polymorphism information content (PIC) was equal to 31.5. The minimum amount of PIC for primer (AG) 8T was equal to 24.5 and maximum amount of PIC for primer GGGC (GA) 8 was equal to 038/5. The maximum amount of MI for primer (AG) 8YT was equal to 21.82, therefore it is the most proper primer for further studies. Results showed high variation among and between genotypes and there is the chance of selection and modification. | ||
| Keywords | ||
| Molecular markers; Principal coordinate analysis; Marker indicator; Polymorphism information content; Analysis of molecular variance | ||
| References | ||
|
Afshar, I. 1991. Traditional Gift of Iranian People. Homa Publication: 302pp.
Aghaabasi, K. and Baghizadeh, A. 2012. Investigation of genetic diversity in flixweed(Descurainia sophia) germplasm from Kerman province using inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers. African Journal of Biotechnology, 11: 10056-10062.
Amin, GH. 2000. Traditional Herbal Drugs in Iran. Search and Education Assistance of Health Ministry Publication. Sari: pp. 1-9.
Bekker, N. P., Ulchenko, N. T. and Glushenkova, A. I. 2005. Lipids from Descurainia sophia seeds. Chemistry of Natural Compounds, 41: 346-347.
Blair, M. W., Panaud, O. and McCouch, S. R. 1999. Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theoretical Appled Genetics, 98: 780-792.
Daryaei, M. 2007. Treatment Miracles of plant in Iranian Medicine. Safirardehal Publication. First Printing, p. 218.
Doyle, J. J. and Doyle, J. L. 1990. A rapid DNA prepration procedure for fresh plant tissue. Focus, 12: 13-15.
Dublin, S., Lydon-Rochelle, M. and Kaplan, RC. 2000. Maternal and neonatal outcomes after inducation of labor and identified indication. American Journal of Obstetrics and Gynecology, 183(4): 986-94.
Duke, J. A. and Ayensu, E. S. 1985. Medicinal Plants of China. Reference Publications Inc., ISBN 0-917256-20-4, p. 705.
Everitt, J. H., Lonard, R. L. and Little, C. R. 2007. Weeds in South Texas and NorthernMexico. Lubbock: Texas Tech University Press. ISBN 0-89672-614-2.
Excoffier, L., Smouse, P. E. and Quattro, J. M. 1992. Analysis of Molecular Variance inferred frommetric distances among DNA haplotype: application to human mitochondrial DNA restrictiondata. Genetics, 131: 479-491.
Fang, D. Q. and Roose, M. L. 1997. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theoretical Applied Genetics, 95: 408-417.
Farsi, M. and Zolala, J. 2003. Principle of plant biotechnology. Translate. Mashhad University Publication, p. 495.
Gonzalez, A., Coulson, A. and Brettell, R. 2000. Development of DNA markers (ISSR) in mango. Acta Horticulture, 575: 139-143.
Gruzdiene, D. and Bagdonaite, K. 2003. Traditional and nontraditional oil plants grown in Lithuania: Chemical characteristics and stability of edible oil. The IIth Lithuania-Germany oily plants day. Lithuanian Academy of Veterinary, Kaunas, pp. 31-37.
Gupta, P. K. and Varshney, R. K. 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica, 113: 163-185.
He, F., Kang, D., Ren, Y., Qu, L. J., Zhen, Y. and Gu, H. 2007. Genetic diversity of the natural populations of Arabidopsis thaliana in China. Heredity, 99: 423-431.
Huang, J. C. and Sun, M. 2000. Genetic diversity and relationships of sweet potato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theoretical Applied Genetics, 100: 1050-1060.
Iqbal, Z. and Saddiqi, H. 2011. Nuts and Seeds Used in Health and Disease in Pakistan-Chapter, 10: 93-100.
Khezri, S. SH. 2003. Herb Culture (Properties of fruits, herbs and vegetables). Print Rostamkhani, P. 572.
Kresovich, S. and McFerson, R. 1992 .Assessment and management of plant genetic diversity: considerations of intra- and interspecific variation. Field Crops Research, 29(3): 185-204.
Lu, X., Liu, L., Gong, Y., Zhao, L., Song, X. and Zhu, X. 2009. Cultivar identification and genetic diversity analysis of broccoli and its related species with RAPD and ISSR markers. Scientia Horticulturae, 122: 645-648.
Marsalkiene, N., Sliesaravicius, A., Karpaviciene, B. and Dastikaite, A. 2009. Oil content and fatty acid composition of seeds of some Lithuanian wild crucifer species. Agronomy Research, 7: 654-661.
Mattioni, C., Casasoli, M., Gowzales, M. and Ipinza, R. 2002. Comparsion of SSR and RAPD to characterize three Chilean Nothofagous Species. Theoretical and Applied Genetics, 104: 1064-1070.
Moreno, S., Martin, J. P. and Ortiz, J. M. 1998. Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm. Euphytica, 101: 117-125.
Nasiri, A. 2011. Genetic diversity of some commercial varieties of sugar beet and Iranian accessions of Beta vulgaris subsp. Maritima using molecular markers. Thesis Submitted for Master of Science in Field of Biotechnology. Faculty of Agriculture. Bu-Ali Sina University, 152pp.
Peng, L., Yi, Y., Fu-li, G. and Ze-qu, L. 1997. A preliminary study on the introduction of Descurainia sophia, an oil plant species for industrial uses. Acta Botanica Sinica, 39(5): 447-479.
Rashed. Mohassel, M. H., Najafi, H. and Akbarzadeh, M. D. 2000. Biology and Weed Control. Published Ferdowsi University of Mashhad, 404pp.
Saki, S., Bagheri, H., Deljou, A., Zeinalabedini, M. 2016. Evaluation of genetic diversity amongst Descurainia sophia L. genotypes by inter-simple sequence repeat (ISSR) marker. Physiology and Molecular Biology of Plants, 22(1): 97-105.Shi, A., Kantartzi, S., Mmbaga, M. and Chen, P. 2010. Development of ISSR PCR markers for diversity study in dogwood (Cornus sp.). Agriculture and Biology Journal of North America, 1(3): 189-194.
Sun, K., Li, X., Liu, J. M., Wang, J. H., Li, W. and Sha, Y. 2005. A novel sulphur glycoside from the seeds of Descurainia sophia (L.). Journal of Asian Natural Products Research, 7(6): 853-856.
Tkachuk, R. and Mellish, V. J. 1977. Amino acid and proximate analyses of weed seeds. Canadian Journal of Plant Science, 57(1): 243-249.
Wanntorp, L., Kocyan, R., Van, D. and Renner, S. 2006. Toward a monophyletic Hoya (Marsdeniease, Apocynaceae) inferences from the Chloroplasmtrn L region and the rbcL-atpB spacer. Systematic Botany, 31: 586-589.
Zargai, H. 1997. Pharmaceutical plant. Tehran University Puplication, 1(7): 213-214.
Zhang, Z. P. 2003. Development of chemical weed control and integrated weed management in China. Weed Biology and Management, 3(4): 197-203.
Zietkiewicz, E., Rafalski, A. and Labuda, D. 1994. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification. Genomics, 20(2): 176-183. | ||
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