Statistics
| Number of Journals | 22 |
| Number of Issues | 501 |
| Number of Articles | 5,252 |
| Article View | 9,906,545 |
| PDF Download | 6,602,421 |
Generation of Composite Plants in Licorice (Glycyrhiza glabra L.) Using Agrobacterium rhizogenes | ||
| فناوری زیستی در کشاورزی | ||
| Article 1, Volume 7, Issue 1, June 2016, Pages 1-7 PDF (568.1 K) | ||
| Document Type: research | ||
| DOI: 10.22084/ab.2016.1783 | ||
| Authors | ||
| Khosro Piri* 1; Khadigeh Sepahvand2; Asghar Mirzaie Asl3 | ||
| 1Associate Professor, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan | ||
| 2M.Sc. Student, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan | ||
| 3Assistant Professor, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan | ||
| Abstract | ||
| Licorice is one of the most important medicinal plants in the world. Licorice root contains active triterpene saponins. Glycyrrhizin and its hydrolysis products are important in the pharmaceutical industry and food products. Many higher plants are sensitive to Agrobacterium rhizogenes and their inoculation lead to hairy roots induction. Plant secondary metabolite levels may be enhanced by hairy root culture. Composite plants are an alternative to tissue culture-derived hairy roots cultures for transgenic studies. In this study, licorice composite plants using Agrobacteriumrhizogenes strain AR15834 carrying the binary vector PBI121 with GUS reporter gene were obtained. 14-day-old seedlings grown in pots and sterile seedlings 3-5 days of Glycyrrhiza glabra were cut from internodes and were inoculated with bacterial suspensions. 50-60% of 14-day pot seedlings and 80-90% of 3-5 day seedlings produced hairy roots. Hairy roots of appeared from Rock wool pieces 3-4 weeks after inoculation. The hairy roots were tested using a histochemical GUS assay. GUS gene expression was demonstrated in some of hairy roots but in non-transgenic hairy root GUS gene activity was not observed. | ||
| Keywords | ||
| Glycyrrhiza glabra; Composite plants; Agrobacterium rhizogenes; Rockwool; Transgenic hairy roots | ||
| References | ||
|
Barker, D. G., Chabaud, M., Dernier, A. B. and Taylor, O. Y. 2006. Agrobacterium rhizogenes-mediated root transformation. Medicago truncatula handbook. 99 pp.
Cai, G., Li, G., Ye, H. and Li, G. 1995. Hairy root culture of Artemisia annua L. by Ri plasmid transformation and biosynthesis of artemisinin. Chin Journal Biotechnology, 11: 227-35.
Chilton, M. D., Tepfer, D. A. and Petit, A. 1982. Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plants root cells. Nature, 295: 432-434.
Christopher, G., Taylor, C. G., Fuchs, B., Collier, R. and Kevin L. W. 2006. Generation of composite plants using Agrobacterium rhizogenes. Methods in Molecular Biology, 343: 155-168.
Deng, Y., Mao, G., Stutz, W. and Yu, O. 2011. Generation of composite plants in Medicago truncatula used for Nodulation Assays Journal, (49), e2633, DOI: 10.3791-2633.
Giri, A. and Narasu, M. L. 2000. Transgenic hairy root: recent trends and applications. Biotechnology Advances, 18(1): 1-22.
Hamill, J. D., Parr, A. J., Rhodes, M. J. C., Robin, R. G. and Walton, N. J. 1987. New routes to plant secondary products. Biotechnology, 5: 800-804.
Hansen, J., Jorgensen, J. E., Stougaard, J. and Marcker, K. A. 1989. Hairy roots a short cut to transgenic root nodules. Plant Cell Report, 8: 12-15.
Hayashi, H., Kosyan, A. and Kaufman, P. 2009. Molecular biology of secondary metabolism: Case study for Glycyrrhiza plants. Recent Advances in Plant Biotechnology, 89-103.
Hong-yu, L. U., Jing-Mei, L. and Hai-Chao, Z. 2008. Ri-Mediated transformation of Glycyrrhiza uralesis with a squalene synthase gene (GUSQS1) for production of Glycyrrhizin. Plant Moleculor Biology, 26: 1-11.
Jefferson, R. A. 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Molecular Biology Reporter, 5: 387-405.
Kitagawa, I., Kang1, V. R., Anbazhagan1, X. L., You1, H. K., Moon, J. S., Yi and Choi, Y. E. 2002. Licorice root a natural sweetener and an important in gradient in Chinese medicine. Pure Apply Chemistry, 74: 1189-1198.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
Nader, B. L., Taketa, A. T., Pereda-Miranda, R. and Villarreal, M. L. 2006. Production of triterpenoids in liquid-cultivated hairy roots of Galphimia glauca. Planta Medica, 72: 842-844.
Nermin, G. and Tijen, T. 1999. Expressionand inheritance of GUS in transgenic tobacco plants. Turkish Journal of Botany, 23: 297-301.
Sevon, N., Oksman, C. and Kirsi, M. 2002. Agrobacterium rhizogenes mediated transformation: Root cultures as a source of alkaloids. Planta Medica,68: 859-868.
Shirazi, Z., Piri, Kh., Mirzaie Asl, A. and Hasanloo, T. 2012. Glycyrrhizin and isoliquiritigenin production by hairy root culture of Glycyrrhiza glabra. Journal of Medicinal Plants Research, 6(31): 4640-4646.
Soleimani, T., Keyhanfar, M., Piri, Kh. and Hasanloo, T. 2012. Hairy root induction in burdock (Arctium lappa L.). Medicinal Plants Journal, 11(4): 176-184.
Tripathi, L. and Tripathi, J. N. 2003. Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research, 2: 243-253.
Vergauwe, A., Van geldre, E., Inze, D., Vanmontagu, M. and Van den, E. 1998. Factors influencing A. tumefaciens mediated transformation of Artemisia annua L., Plant Cell Reports, 18(1-2): 105-110.
White, F. F. and Nester, E. W. 1980. Hairy root: Plasmid encodes virulence TrqKts in Agrobacterium rhizogenes. Journal of Bacteriology, 141(3): 1134-1141. | ||
|
Statistics Article View: 1,755 PDF Download: 1,309 |
||