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Molecular Detection of Mycoplasma in Milk Samples of Goat Herds in Borujerd County | ||
| Avicenna Veterinary Research | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 20 آبان 1404 | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22084/avr.2025.31600.1020 | ||
| نویسندگان | ||
| Sanaz Bakhshi1؛ Pezhman Mahmoodi* 2؛ Morteza Yavari3؛ Abdolmajid Mohammadzadeh2 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran, | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran | ||
| 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran | ||
| چکیده | ||
| Mycoplasmas are among the smallest bacteria which are responsible for eco- nomically signi cant infections in small ruminants. This study aimed to detect Mycoplasma in goat milk samples from Borujerd County, Iran. Ninety-six milk samples were collected from seven regions and screened for subclinical mastitis using the California Mastitis Test (CMT) and somatic cell count (SCC). Thereafter, total DNA was extracted, and Mycoplasma detection was performed by a PCR assay using genus-speci c primers. High SCC and CMT scores were observed in 7.3% and 31.25% of samples, respectively. PCR anal- ysis con rmed Mycoplasma infection in 5.2% (n=5) of the milk samples. The presence of Mycoplasma infection in goat herds in Borujerd county highlights the need for breeders and veterinary supervisors to take effective measures to control and prevent these infections. Besides, these ndings suggest PCR as- say as a rapid and reliable method for identifying Mycoplasma in milk samples. | ||
| کلیدواژهها | ||
| Mycoplasma؛ Milk؛ Goat؛ PCR | ||
| مراجع | ||
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