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Usage of the Cell Dissociation Technique in Reproductive Studies; Study on Mechanical Cell Dissociation in Male Mice in Maturity Course | ||
| Avicenna Veterinary Research | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 شهریور 1404 | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22084/avr.2025.31451.1013 | ||
| نویسندگان | ||
| arash younesi* 1؛ Nasser Mahdavi Shahri2؛ Roya Lari3؛ Hassan Morovvati4 | ||
| 1Ph.D Candidate, Department of Basic Science, Faculty of Veterinary Medicine University of Tehran. Tehran, Iran. | ||
| 2Professor, Department of Biology, Faculty of Biology Science, Kavian Institute of higher Education, Mashhad, Iran. | ||
| 3Associate Professor, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 4Professor, Department of Basic Science, Faculty of Veterinary Medicine University of Tehran. Tehran, Iran. | ||
| چکیده | ||
| Cell dissociation techniques are one of the main methods for separating different cells from various tissues. These techniques consist of two main approaches: chemical and mechanical separation. Each technique has its own advantages and disadvantages, and the most suitable method should be selected. In the chemical method, specific enzymes must be used. In the mechanical method, cells are separated by mechanical force. Normally, in most research studies, the chemical method using specific enzymes is more commonly applied. In this study, 15 mice were used during the maturity phase and were grown under standard conditions. These mice were operated on after 32 days, and the testes were extracted. The cells were dissociated using the mechanical method. Finally, the samples were stained with Giemsa and examined. The advantages of these techniques include the separation of undamaged cells, which were observed in both methods. However, the main focus of this paper was mechanical cell dissociation, which did not damage the primary cells. Moreover, this method can be used for chromatin studies by counting each cell type and separating them to investigate testicular functions. Therefore, using mechanical cell dissociation for the seminiferous tubule wall could be a useful and economical option as a low-cost method for comparative studies involving cells in testicular tissue. The findings suggest that mechanical dissociation provides an efficient and low-cost method for isolating intact testicular cells, making it suitable for histological and functional studies of seminiferous tubules. | ||
| کلیدواژهها | ||
| Cell dissociation؛ Cell dissociation methods؛ Trypsin؛ collagenase؛ Testes tissue cell dissociation | ||
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