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Prevalence of Brucella Contamination in Unpasteurized Dairy Products in Sistan Region, Iran, Using PCR Assay | ||
| Avicenna Veterinary Research | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 02 شهریور 1404 | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22084/avr.2025.31331.1011 | ||
| نویسندگان | ||
| Kamand Mehdipour1؛ Dariush - Saadati* 2؛ Mohsen Najimi3؛ Ali Hosseini4 | ||
| 1DVM graduated, Faculty of veterinary medicine, university of Zabol, Zabol, Iran. | ||
| 2Department of Food Hygiene, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran. | ||
| 3Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran. | ||
| 4Zabol veterinary office, Sistan and Baluchestan provincial veterinary service, Iranian veterinary organization, Zabol, Iran. | ||
| چکیده | ||
| Brucellosis is one of the most widespread zoonotic diseases globally, posing a significant public health and economic burden. It is primarily transmitted to humans through the consumption of contaminated milk and raw dairy products, or by direct contact with infected animals. This study aimed to determine the prevalence of Brucella contamination in unpasteurized dairy products sold in the Sistan region, Iran, utilizing the Polymerase Chain Reaction (PCR) method. A total of 175 unpasteurized traditional dairy samples were collected from various distribution centers in Zabol city. These samples included 35 samples each of milk, yogurt, cheese, buttermilk, and ice cream. DNA extraction was performed using the boiling method. Following DNA extraction, PCR was conducted to detect the presence of Brucella bacteria and identify the species, specifically targeting Brucella abortus and Brucella melitensis. The results revealed that only one sample (0.58% of 175 tested samples) was positive for Brucella. This positive sample was identified as Brucella abortus, and it originated from one of the 35 milk samples analyzed, indicating a prevalence of 2.86% in unpasteurized milk samples. This low prevalence of Brucella infection in the Sistan region, compared to findings from other past studies in the area, is likely attributable to the recent, systematic, and annual free vaccination campaigns against brucellosis in livestock throughout the Sistan region. These findings highlight the effectiveness of ongoing vaccination efforts in reducing the spread of brucellosis in the region. | ||
| کلیدواژهها | ||
| Brucella abortus؛ Brucella melitensis؛ Sistan؛ Unpasteurized dairy products؛ Polymerase Chain Reaction | ||
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آمار تعداد مشاهده مقاله: 9 |
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