فعالیت آنتی‌اکسیدانی سیب

مصطفایی, سجاد; اصغری, محمدرضا; قوستا, یوبرت

doi:10.22084/ppt.2025.30941.2160

اخذ مجوز راه اندازی 5 عنوان نشریه ی جدید از وزارت علوم ، تحقیقات و فناوری

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	فعالیت آنتی‌اکسیدانی سیب "رد‌ دلیشز" تحت محلول‌پاشی قبل از برداشت اسید‌فرولیک و نانوکیتوسان
دوفصلنامه فنآوری تولیدات گیاهی
دوره 17، شماره 2، بهمن 1404، صفحه 13-26 اصل مقاله (752.72 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22084/ppt.2025.30941.2160
نویسندگان
سجاد مصطفایی¹؛ محمدرضا اصغری^* ²؛ یوبرت قوستا³
¹دانشجوی دکتری، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ایران
²استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ایران
³استاد، گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ایران
چکیده
سیب (Malus domestica) به‌دلیل محتوای بالای ترکیبات فنولی و ظرفیت آنتی‌اکسیدانی، اهمیت تغذیه‌ای بالایی دارد. این پژوهش تأثیر کاربرد قبل از برداشت فرولیک‌اسید و نانوکیتوسان را بر کیفیت سیب بررسی کرد. آزمایش به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با چهار تکرار انجام شد. تیمارها شامل نانوکیتوسان (۰، ۵، ۱۰ میلی‌لیتر در لیتر) و فرولیک اسید (۰، 0/5، ۱ میلی‌مولار) بود. نتایج نشان داد که بالاترین غلظت‌های نانوکیتوسان (۱۰ میلی‌لیتر در لیتر) و فرولیک اسید (یک میلی‌مولار) موجب افزایش معنی‌دار فنول‌کل، فلاونوئید‌کل و فعالیت آنتی‌اکسیدانی براساس آزمون DPPH شدند. در مقابل، فعالیت آنزیم پلی‌فنول‌اکسیداز با افزایش غلظت تیمارها کاهش یافت و کمترین مقدار در تیمار ترکیبی نانوکیتوسان (۱۰ میلی‌لیتر در لیتر) و فرولیک‌اسید (0/5 میلی‌مولار) مشاهده شد. بیشترین فعالیت کاتالاز در تیمار فرولیک‌اسید (0/5 میلی‌مولار) بدون نانوکیتوسان ثبت شد، درحالی‌که کمترین فعالیت این آنزیم در تیمار ترکیبی نانوکیتوسان (۱۰ میلی‌لیتر در لیتر) و فرولیک اسید (یک میلی‌مولار) مشاهده شد. با افزایش غلظت فرولیک‌اسید، مقدار گالیک‌اسید کاهش و کلروژنیک‌اسید افزایش یافت، درحالی‌که نانوکیتوسان باعث افزایش گالیک‌اسید و تغییرات مختلف در کافئیک‌اسید شد. همچنین، بیشترین مقدار آپی‌ژنین در ترکیب نانوکیتوسان 10 میلی‌لیتر در لیتر و فرولیک‌اسید یک میلی‌مولار مشاهده شد. استفاده همزمان از نانوکیتوسان و فرولیک‌اسید، به‌ویژه در باغ‌های ارگانیک، روشی مؤثر برای بهبود کیفیت تغذیه‌ای سیب و افزایش ارزش بازارپسندی آن است
کلیدواژه‌ها
پلی‌فنل‌کسیداز؛ فنول‌کل؛ فلاونوئید‌کل؛ کاتالاز

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فعالیت آنتی‌اکسیدانی سیب "رد‌ دلیشز" تحت محلول‌پاشی قبل از برداشت اسید‌فرولیک و نانوکیتوسان