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Histological Characterization of the Brain and Eye in Drosophila Melanogaster: A Model for Neurodegenerative Research | ||
| Avicenna Veterinary Research | ||
| مقاله 5، دوره 1، شماره 1، شهریور 2025، صفحه 27-30 اصل مقاله (1.56 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22084/avr.2025.31018.1005 | ||
| نویسندگان | ||
| Mahsa Hallaj-Salahipour* 1؛ Behnaz Ghorbanzadeh2 | ||
| 1Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| چکیده | ||
| Background: Drosophila melanogaster, a key model in genetic and neurological research, offers advantages due to its short life cycle (10 days), high fertility (400{500 offspring per cycle), and ethical feasibility for experiments. It has been widely used in studies of Alzheimer's, diabetes, and Parkinson's disease, yet limited histological analysis of its head structures has been conducted in Iran. This study aims to characterize its brain and ocular histology to enhance its application in neurodegenerative disease modeling. Material & Methods: Drosophila melanogaster specimens were anes-thetized at temperatures below 4◦ C, then sex-separated under a dissecting microscope. Heads were dissected from the thorax, ensuring optimal penetration of Carnoy's xative. Samples underwent xation, dehydration, paraffin embedding, and serial sectioning. Histological staining was performed, and imaging was conducted using Dino-Lite digital microscopy, processed via Dino Capture software (Version 2). Results: Histological analysis showed that the eye's external surface consists of mosaic-shaped photoreceptor cells, beneath which lies the retinal layer. Below the retina, the lamina was identi ed. The brain contains two hemispheres, each with lamina, medulla, lobula plate, and lobula. Lobular architecture revealed dense neuronal nuclei surrounding each lobe, with interspersed bers and centrally distributed neuroglial matrix. Neuronal ber degeneration led to vacuolated spaces, mainly within lobular cores, indicating neurodegenerative changes. Conclusion: Considering the distinct histological characteristics of Drosophila melanogaster especially the prominent staining properties of its head structures it appears to be a highly suitable laboratory model for studying neurodegenerative diseases and evaluating the impact of various factors on disease induction and recovery. | ||
| کلیدواژه‌ها | ||
| Drosophila melanogaster؛ Eye؛ Brain histology؛ Retinal morphology؛ Hematoxylin-eosin staining | ||
| مراجع | ||
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