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Molecular Detection of Neospora Canium in Bovine Colostrum: A Possible Risk Factor for Neonatal Infection | ||
| Avicenna Veterinary Research | ||
| مقاله 2، دوره 1، شماره 1، شهریور 2025، صفحه 11-16 اصل مقاله (2.11 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22084/avr.2025.30999.1004 | ||
| نویسندگان | ||
| Khadijeh Seydi-Gazafi1؛ Ali Sadeghi-nasab* 2؛ Hossein Rezvan1؛ Mehdi Namavari3؛ Sahar Hamoonnavard1؛ Bahram Dadmehr4 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran | ||
| 2Department of Clinical Medicine, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran | ||
| 3Razi Vaccine and Serum Research Institute, Shiraz, Iran | ||
| 4Department of Basic Sciences, Applied Science Education Center of Farab Amarlou, Jirandeh, Iran | ||
| چکیده | ||
| Neospora caninum is an obligate intracellular parasite that is both vertically and horizontally transmitted in cattle leading to signi cant economic losses in the cattle industry. The aim of this study was to investigate the prevalence of colostrum contamination in Neospora positive cows as a potential route for horizontal transmission. To this end, whole blood and colostrum from 44 fresh cows were analyzed using polymerase chain reaction (PCR) and nested PCR methods to detect the presence of the parasite DNA. In addition, serum samples were tested with a whole cell-based enzyme-linked immunosorbent assay (ELISA) to identify antibodies against the parasite. The results showed that 19 out of 44 colostrum samples (43.18%) contained the parasite genome. In addition, 16 of 18 (88.9%) Neospora -positive cows and 13 of 14 (92.9%) seropositive cows were found to have secreted infected colostrum. The relative risk (RR) of colostrum infection was signi cantly higher in blood PCR-positive cows (RR=7.7, P=0.0002) and seropositive cows (RR=4.6, P<0.0001) compared to uninfected cows. Based on these results, it is suggested that colostrum may be considered as a potential source of horizontal transmission in newborn calves and that methods for removing the infection such as pasteurisation may be required to reduce the risk of transmission. | ||
| کلیدواژه‌ها | ||
| Neospora؛ Colostrum؛ Blood؛ Seropositive؛ PCR | ||
| مراجع | ||
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