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Horizontal Polyacrylamide Gel Electrophoresis by Agarose Slots | ||
| فناوری زیستی در کشاورزی | ||
| Article 4, Volume 6, Issue 1, May 2015, Pages 33-37 PDF (242.63 K) | ||
| Document Type: Research Paper | ||
| Authors | ||
| mohammad hossein safari; Asghar Mirzaie-asl* | ||
| Abstract | ||
| Horizontal submerged agarose gel electrophoresis of nucleic acids is a basic tool of modern molecular biology. This method is quick and easy and the use of that does not require too much skills. Separation of small fragments of DNA and molecular proteins are not possible with agarose gel efficiently, so that acrylamide gel is used for the above mentioned aims. Vertical polyacrylamide gel electrophoresis, which is costly, time consuming with hard handling. Recently, a method of horizontal polyacrylamide gel electrophoresis was introduced which requires especial equipment. In this study, we used the agarose electrophoresis tank with changes for small DNA fragments of SSR markers separation by horizontal polyacrylamide gel electrophoresis. In this method, horizontal electrophoresis performed using by a two-part gel prepared with agarose and polyacrylamide. The results showed the very sharp bands and in addition, in this electrophoresis method air bubbles not formed and cause reduction rupturing in the gel by glasses separation. New devised electrophoresis system can be applied for two aims, agarose and polyacrylamide gels electrophoresis. | ||
| Keywords | ||
| Horizontal electrophoresis; Polyacrylamide gel; Agarose gel | ||
| References | ||
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Izzo, V., Costa, M. A., Fiore, Rd., Duro, G., Bellavia. D., Cascone, E., Colombo, P., Gioviale, M. C. and Barbieri, R. 2006. Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels. Immunity and Ageing, 3(7): 1-5.
Marcheylo, B. A., handel, K. A. and Mellish, V. J. 1989 Fast horizontal Sodium Dodecyl Sulfate gradient polyacrylamide gel electrophoresis for rapid wheat cultivar identification and analysis of high molecular weight glutenin subunits. Cereal Chemistry, 66(3): 186-192.
Sambrook, J., Fritsch, E. F. and Maniatis, T. 2001. Molecular Cloning: A Laboratory Manual. 3nd edition. Cold Spring Harbor Laboratory, 545 p.
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