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Isolation and Characterization of a Chitinase Gene Exhibiting Antifungal Activity Against Fusarium solani f. sp. melongenae from an Iranian Streptomyces strain | ||
| فناوری زیستی در کشاورزی | ||
| Article 5, Volume 1, Issue 2, December 2010, Pages 39-47 PDF (565.58 K) | ||
| Document Type: Research Paper | ||
| Authors | ||
| Azam Baharlouei1; Gholam Reza Sharifi-sirchi* 2; Gholam Hossein Shahidi Bonjar3 | ||
| 1Graduate Student , Department of Agricultural Biotechnology, Faculty of Agricultur, Shahid Bahonar University of Kerman | ||
| 2Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultur, Shahid Bahonar University of Kerman | ||
| 3Professor, Department of Plant Pathology, Faculty of Agricultural Sciences, Shahid Bahonar University of Kerman | ||
| Abstract | ||
| Fusarium solani f. sp. melongenae, the causal agent of egg plant wilt disease, has a wide host range which causes significant reduction in yield of eggplant (Solanum melongena). In a survey for finding biocontrol agents, 110 Actinomycetes strains were isolated from agricultural soils of Kerman province. Amony isolated strains 18 strain showed high level of chitinase activity Streptomyces griseus. Streptomyces isolate 401 significantly reduced the incidence of disease. Some biological and physiological characteristics of this isolate were investigated under green house condition. Moreover, the genomic DNA of this isolate was extracted by useing of CTAB method and a full chitinase gene (876 bp) was cloned and sequenced. The results showed high similarity between obrouned scyuence and seyuence of chitinasegene 19 fmily from. | ||
| Keywords | ||
| Biological control; Streptomyces; Chitinase; Fusarium solani | ||
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